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Paper | Regular issue | Vol 91, No. 3, 2015, pp.515-525
Published online, 30th January, 2015
DOI: 10.3987/COM-14-13152
Development of a Probe Containing Novel Fluorescent Tricyclic-Nucleoside Analogs for Detecting Single Nucleotide Polymorphisms

Ayako Moriya, Tokimitsu Ohki, Aya Hayai, and Yoshihito Ueno*

*Faculty of Applied Biological Sciences, Gifu University , 1-1 Yanagido, Gifu, Gifu 501-1193, Japan

Abstract

Here we report the synthesis of nucleoside analogs containing several aryl groups at the 6-position of the fluorescent tricyclic base, 8-amino-3-(2,3-dihydroxypropyl)imidazo[4′,5′:5,6]pyrido[2,3-d]pyrimidine (1), to expand the fluorescence color range of single nucleotide polymorphism (SNP)-detecting probes. The identity of the RNA target bases could be determined using probes containing an analog with a phenyl group at the 6-position of 1.