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Paper | Regular issue | Vol 89, No. 7, 2014, pp.1585-1593
Published online, 12th May, 2014
DOI: 10.3987/COM-13-12882
Two New Clerodane Diterpenoids from Croton crassifolius

Wei-Huan Huang, Guo-Qiang Li, Jia-Gui Li, Xia Wu, Wei Ge, Hau Yin Chung, Wen-Cai Ye, Yao-Lan Li,* and Guo-Cai Wang*

*Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, 601 West Huangpu Avenue, Guangzhou, 510632, China


The root of Croton crassifolius was evaluated for its anti-angiogenic properties and anti-angiogenic components. The ethanol extract of the herb and its successive four subfractions were investigated in zebrafish model by quantitative endogenous alkaline phosphatase assay. Both petroleum ether (PE) and ethyl acetate fractions (EtOAc) showed potent anti-angiogenic activities. Two new clerodane diterpenoids, crassifolin I (1) and crassifolin H (2) were isolated from EtOAc fraction. Their structures were elucidated by extensive spectroscopic methods (IR, UV, HRESIMS, 1D and 2D NMR), and compound 1 was confirmed by single-crystal X-ray diffraction analysis. The further activities study showed that crassifolin H possessed anti-angiogenic activity by reducing vessel formation to 59.3% of the control value at concentrations of 15 μg/mL.