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■ Synthesis of Imidazole C1- and C3-Ribonucleoside Phosphoramidites for Probing Catalytic Mechanism in Ribozyme

Shinya Harusawa,* Kensuke Fujii, Masayoshi Nishiura, Lisa Araki, Yoshihide Usami, Zheng-yun Zhao, and David M. J. Lilley

*Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan

Abstract

Synthesis of C4-linked imidazole N-pivaloyloxymethyl (POM)-2’-O-cyanoethylated (CE) C1- and C3-ribonucleoside phosphoramidites 1a and 1b is described. These phosphoramidite products were incorporated into RNA sequence through solid phase phosphoramidite approach, providing RNA with imidazole linked through different length to sugar residue, to study the mechanism of a ribozyme.