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Note | Regular issue | Vol 81, No. 11, 2010, pp.2599-2608
Published online, 28th September, 2010
DOI: 10.3987/COM-10-12031
Synthesis of Readily Accessible BODIPY-Based Fluorescence Derivatization Reagents for Amines

Ryota Saito* and Eri Ito

*Toho University, 2-2-1, Miyama, Funabashi, Chiba 274-8510, Japan

Abstract

Two BODIPY-based fluorescence derivatization reagents (FDRs) for amines were synthesized in a simple manner with practically acceptable yields via five steps. Both FDRs showed prompt reactivity, even against sterically hindered amines, to afford the corresponding FDR-labeled products in good yields. The solvent effect upon the fluorescence intensity of the derivatized amine was also investigated in a methanol-water binary solvent system to demonstrate that the strong fluorescence intensity was maintained in the solvent with a 0-60% water ratio. The detection limits of the new FDRs were estimated to be 20 fmol, which makes it much more sensitive than commercially available FDRs.