Online article for Non-subscribers

Pay per view

Heterocycles has a pay-per-view service for Non-subscribers.
You will be able to directly purchase the full text article through PayPal.
Your purchased Paper can be downloaded after the payment is completed.
An e-mail will be sent the URL to download the paper.
If you have any questions, please contact:

Price: ¥ 4,400 (Yen only)
Period: This Article can be accessed for 7 days.

Communication | Special issue | Vol 5, No. 1, 1976, pp.347-353
Published online, 1st January, 1970
DOI: 10.3987/S-1976-01-0347
β-Lactones as Reagents in Protein Chemsitry. The Inactivation of Pepsin with β-Propiolactone

Richard N. Armstrong and Emil Thomas Kaiser*

*Departments of Biochemistry and Chemistry, University of Chocago, Chocago, Illinois 60637, U.S.A.


Treatment of pepsin with β-propiolactone results in the alkylation of 1-2 methionine residues and the esterification of an aspartate residue. The modified enzyme is characterized by a marked (20-fold) decrease in catalytic efficiency toward benzyloxycarbonyl-histidyl-p-nitrophenyl-alanyl-phenylalanyl methyl ester. The results suggest that the aspartate alkylated is in the active site.