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■ The Early Stages of Papaver Alkaloid Biosynthesis. Cell-Free Synthesis of Benzykisoquinolines

A. Ian Scott,* Siu-Leung Lee, and Toshifumi Hirata

*Department of Chemistry, Texas A & M University, College Station, Texas 77843-3255, U.S.A.

Abstract

A cell-free system of Papaver somniferum has been isolated, capable of synthesizing norlaudanosoline-1-carboxylic acid, dehydronorlaudanosoline, and norlaudanosoline from 3,4-dihydroxyphenethylamine (dopamine) and 3,4-dihydroxyphenylpyruvic acid. The enzyme activity lies in a Triton X-100 solubilized protein fraction.