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■ Studies on the Index Compounds for HPLC Analysis of Glycyrrhiza uralensis

Makio Shibano, Ayako Henmi, Yoshihiro Matsumoto, Genjiro Kusano,* Toshio Miyase, and Yoshio Hatakeyama

*Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan

Abstract

HPLC of the AcOEt extracts of subterranean (roots or stolons) samples of Glycyrrhiza uralensis showed two types of the reproducible chromatograms distinct from those of other species (G. glabra, G. pallidiflora, G. echinata, G. macedonica). As index compounds for HPLC of G. uralensis, two new compounds, named glyurallins A and B, were isolated and their structures were difined, in addition to identification of nine compounds to glycycoumarin, semilicoisoflavone B, dehydroglyasperin C, licoisoflavanone, glycyrol, glycyrin, gancaonin N, licoricidine and 8-γ,γ-dimethylallylwighteone.