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Paper | Special issue | Vol 40, No. 1, 1995, pp.425-440
Published online, 1st January, 1970
DOI: 10.3987/COM-94-S73
Purification and Characterization of a Cytochrome P450 Enzyme from Pig Liver, Catalyzing the Phenol Oxidative Coupling of (R)-Reticuline to Salutaridine, the Critical Step in Morphine Biosynthesis

Tobias Amann, Peter H. Ross, Hoon Huh, and Meihart H. Zenk

*Lefrstuhl für Pharmazeutische Biologie, Universität München, Karlstr. 29, 80333 München, Germany


The cytochrome P450 enzyme catalyzing the conversion of (R)-reticuline to salutaridine in the presence of cytochrome c reductase, NADPH and O2 was purified to homogeneity from pig liver and shown to be a highly regio- and stereoselective enzyme. This finding supports the view that the occurrence of morphine in mammals is of endogenous and not of dietary origin.