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Communication | Special issue | Vol 37, No. 2, 1994, pp.729-733
Published online, 1st January, 1970
DOI: 10.3987/COM-93-S103
Kinetic Study of the Reduction of Methemoglobin with Ascorbate Using a COBAS-FARA Centrifugal Analyzer

Kilian Dill* and Evelyn L. McGown

*Molecular Device Corporation, 4700 Bohannon Drive, Menlo Park, CA, 94025, U. S. A.


The reduction-kinetics of methemoglobin with ascorbic acid were monitored using a COBAS-FARA centrifugal analyzer. The data from the initial rates for the reduction of hemoglobin were monitored as a function of the ascorbate concentration. The results show a first order dependence of the reaction rates on the ascorbate concentration and the rate constant was found to be 1.34 x 10-2 s-1 M-1. Ascorbic acid is a one electron donor in this process and consequently forms a stable radical. The presence of oxyhemoglobin interferes with the reduction of methemoglobin by ascorbate.