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Communication | Regular issue | Vol 27, No. 3, 1988, pp.621-628
Published online, 1st January, 1970
DOI: 10.3987/COM-87-4410
Alkaloid Production in Catharanthus roseus (L.) G. Don Cell Cultures. XV. Synthesis of Bisindole Alkaloids by Use of Immobilized Enzyme Systems

James Peter Kutney,* Camille Andre Boulet, Lewis Siu Leung Choi, Wlodzimierz Gustowski, Michael McHugh, Jun Nakano, Tamotsu Nikaido, Hiroki Tsukamoto, Gary Maxwell Hewitt, and Roger Suen

*Department of Chemistry, The University of British Columbia, 2036 Main Mall, Vancouver, B.C., V6T 1Z1, Canada

Abstract

Affinity gel bound enzyme systems obtained from Catharanthus roseus cell cultures were employed to establish a high yielding enzymatic system for the coupling of catharanthine (1) and vindoline (2) to 3’,4’-anhydrovinblastina (3) and leurosine (4). Long term preserved activity can be prerenred by this technique, an aspect which may prove important in subsequent utilization of such systems in large scale production.