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Paper | Special issue | Vol 56, No. 1-2, 2002, pp.341-352
Published online, 1st January, 1970
DOI: 10.3987/COM-01-9268
Biosynthesis of 2,3-Dihydroxybenzoic Acid in Transgenic Catharanthus roseus Cell Cultures Overexpressing Isochorismate Synthase

Retno A. Budi Muljono, Julian R. Talou, Johannes J. C. Scheffer, and Robert Verpoorte*

*Division of Pharmacognosy, Leiden/Amsterdam Center for Drug Research, Leiden University, Gorlaeus Laboratories, P.O.Box 9502, 2300 RA Leiden, The Netherlands

Abstract

The involvement of isochorismate synthase (EC 5.4.99.6) in the biosynthesis of 2,3-dihydroxybenzoic acid (2,3-DHBA), was confirmed by the incorporation of [1-13C]pyruvate in 2,3-DHBA in a transgenic C. roseus cell line constitutively overexpressing the C. roseus plant ics-gene. The label was mainly in the carboxyl group and at C-3 and C-4, which is consistent with isochorismate as intermediate.